Peripheral Blood Mononuclear Cells
Peripheral blood mononuclear cells are a mixed immune-cell compartment commonly used for accessible blood-based immune profiling. In this paper workspace, PBMC notes should distinguish measurements of cell composition from measurements of cell state or function.
Cell Compartments To Track
- T cells.
- B cells.
- NK cells.
- Monocytes.
- Dendritic-cell-like populations, depending on assay and annotation strategy.
Measurement Modes
- Flow or mass cytometry for cell proportions and activation markers.
- Bulk transcriptomics for aggregate PBMC expression signatures.
- Single-cell transcriptomics for cell-type-specific expression and cell-state inference.
- Paired single-cell V(D)J profiling for TCR/BCR repertoire features, such as the cytotoxic T-cell clonality and BCR isotype analyses in Gu et al. 2024.
- Functional stimulation assays for cytokine or activation responses.